【摘要】 冬小麦品种复壮３０中具有一对抗白粉病隐性基因，该基因对我国流行的白粉病生理小种表现为高抗或免疫，虽尚未定名，却已受到国内外的重视。我们分别以复壮３０、感病品种农大０１５、京花一号的ＤＮＡ为模板，采用３００个ＲＡＰＤ 引物（ＵＢＣ４００－ＵＢＣ６９９）进行ＰＣＲ扩增，其中ＵＢＣ４０５（ＣＴＣ ＴＣＧ ＴＧＣ Ｇ）可在抗、感品种之间扩增出一多态性片段（６２８ｂｐ），定名为ＵＢＣ４０５－６２８。通过对Ｆ２群体的ＲＡＰＤ分析，证明这条带与复壮３０的抗白粉病基因有连锁关系，可作为该基因的分子标记。更多还原

【Abstract】 Powdery mildew (Blumeria graminis CD f. sp. tritici) is an important fungal disease in wheat. The most effective method to control the disease is to develop host resistance.Mapping Pm genes permits marker assisted selection in wheat improvement programs. A recessive Pm gene of a wheat variety-Fu Zhuang 30 confers a broad spectrum resistance to powdery mildew in China. Here, we reported a RAPD marker linked to this gene. A 628 bp fragment was specifically amplified with RAPD primer UBC405 (CTCTCGTGCG) in Fu Zhuang 30, but not in susceptible cultivars Nong Da 015 and Jing Hua No. 1. With bulk segregant analysis of 101 F2 progenies of Nong Da 015/ Fu Zhuang 30 and 62 F2 progenies of Jing Hua No. 1/Fu Zhuang 30 crosses, it is evidenced that UBC405-628 linked to Pm gene of Fu Zhuang 30 with a distance of 13 cM and a LOD value of 7.4. Even the genetic distance is 13 cM, before closer one be found, it can be used in molecular marker assisted selection of wheat breeding program.更多还原