【摘要】 用水稻抗白叶枯病基因近等基因系材料和转基因系材料，研究了水稻—白叶枯病菌互作中，水稻防卫基因ｐａｌ与ｃｈｓ的转录特征。Ｎｏｒｔｈｅｒｎ检测结果显示，在与白叶枯病菌非亲和性互作中，水稻ｐａｌ基因和ｃｈｓ基因的转录均受到白叶枯病菌的诱导；而亲和性互作中，水稻中这两种基因的转录均不受到诱导或只是受到很微弱的诱导。表明由ｐａｌ和ＣＨＳ调控的苯丙烷核心反应的启动，产物的合成，有助于增强水稻对白叶枯病菌的抗性。实验结果显示：互作中水稻ＰＡＬ及ＣＨＳ编码基因是否受到白叶枯病菌的诱导，不仅与水稻中抗病基因有关，还与抗病基因的种类有关。表明水稻ＰＡＬ及ＣＨＳ编码基因在互作中是否受到诱导转录，取决于其水稻功能性抗病基因产物对白叶枯病菌的专化性识别。更多还原

【Abstract】 The transcripts of pal and chs genes, two defense gense encoding respectively phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) of the near-isogenic lines and transgentic lines for bacterial blight resistance of rice were detected during interaction with Xanthomonas oryzae pv. oyrzae (Xoo). Northern blot analysis showed that the accumulation of pal and chs gene transcripts was induced by the incompatible races, not the compatible races of Xoo, and the expression of these genes were coordinated. The results suggested the activation of PAL and CHS-mediated phenylpropanoid pathway and induction of product synthesis could enhance resistance of rice to Xoo infection. The results also indiacted the initiation of pal and elfs gene transcription of rice during the interaction with Xoo depended not only on the presence of functional resistance gene, but also the kinds of resistance genes. The findings of this study demonstrated that the specific recognition mediated by the bacterial blight resistance genes is necessary for induction of pal and chs gene transcriptions during the interactions between rice aug Xoo.更多还原