【摘要】 报道了斜纹夜蛾核多角体病毒（Ｓｐｏｄｏｐｔｅｒａ　ｌｉｔｕｒａ　ｎｕｃｌｅｏｐｏｌｙｈｅｄｒｏｖｉｒｕｓ， ＳｐｌｔＮＰＶ）基因组ＤＮＡＸｂａＩ２．０ｋｂ片段的核苷酸全序列，该片段包括三个完整的读码框（ｏｐｅｎ ｒｅａｄｉｎｇ ｆｒａｍｅ， ＯＲＦ），即ＯＲＦ１、ＯＲＦ２、ＯＲＦ３及一个不完整的读码框ＯＲＦ４－ｐａｒｔ。ＯＲＦ１长４７７个核苷酸，可编码１５８个氨基酸的蛋白质，分子量１８．０８ ｋＤ，它与ＡｃＭＮＰＶ ＯＲＦ５１在同源区域内蒙基苷有１８％相同；ＯＲＦ２长５７０个核苷酸，可编码１８９个氨基酸的蛋白质，分子量为２１．６４ｋＤ，它与ＬｄＮＰＶｈｅｌｉｃａｓｅ２基因在同源区域内氨基酸有２２％相同；ＯＲＦ３长１６５个核苷酸，可编码５４个氨基酸的蛋白质，分子量为６．２３ｋＤ，它与已知的杆状病毒基因没有同源性，可能为杆状病毒的一个新基因；不完整的ＯＲＦ４－ｐａｒｔ长５５０个核苷酸，可编码１８３个氨基酸，它编码的氨基酸序列与ＡｃＭＮＰＶ 几丁质酶Ｃ端的氨基酸序列有较高的同源性，相同的氨基酸占５６％。在ＯＲＦ１、 ＯＲＦ２起始密码上游有什状病毒早期后动于基序ＣＡＧＴ，在ＯＲＦ３起始密码上游则有杆状病毒晚期启动子基序ＴＡ?更多还原

【Abstract】 A 2 059 bpXbal fragment of the Spodoptera litura nucleopolyhedrivirus (SpltNPV) genome was sequenced, in which three complete open reading frames (ORFs), ORFI, ORF2, ORF3, and one partial ORF, ORF4-part, were found. ORFI is 477 nucleotides long, encoding a putative protein of 158 amino acids with 18.08 kD, and 18 % homologous to AcMNPV ORF51. ORFZ is 570 nucleotides long, encoding a putative protein of 189 amino acids with 21.64 kD, and 22 % homologous to LdNPV HelicaseZ in the homologous region. ORF3 is 165 nucleotides long, encoding a putative protein of 54 amino acids with 6.23 ac, and no homologous tO the known baculovirus genes, suggesting that it should be a novel gene. The Oar4-part is 550 nucleotides long, encoding 183 amino acids, and highly homologous to the C-terminus of the AcMNPV chitinase gene with the identity of 56 %. The baculovirus early promotcr motifs CAGT for ORF1 and ORF2 and the late promoter motif Ti AG for ORF3 were found in the 5 1 noncoding regions of these ORFs. The polyadenylation signals AATAAA are presented in the downstream of the translation stop codon of ORF1, ORF2 and ORF4-part. The organization based on these ORFs found in SpltNPV is different from that of AcMNPV更多还原