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水稻细菌性条斑病抗性与过氧化物酶同工酶关系的初步研究
Preliminary Study on the Relationship Between Resistance to Bacterial Leaf Streak and Isoperoxidases in Rice
【摘要】 以水稻抗、感细菌性条斑病亲本杂交F2代构建的抗、感近等基因集团为材料,研究了接菌前后细菌性条斑病抗性与过氧化物酶活性及其同工酶酶谱的关系。结果表明,细菌性条斑病对抗病和感病水稻叶片过氧化物酶活性的影响不同,尽管两者酶活性都存在先降低后升高的过程,但感病植株酶活性上升的时间早于抗病植株。过氧化物酶同工酶谱带的差异主要表现在等电点(pI)为4.2~5.3之间的酶带,暗示细菌性条斑病菌能诱导过氧化物酶的某些等位基因的表达,表现为多条同工酶带的变化。本文还对研究植物抗病性与同工酶关系的主要技术环节进行了讨论。
【Abstract】 The relationship between resistance to bacterial leaf streak and peroxidases activities and isoperoxidase zymograms during infection by Xanthomonas campestris pv. oryzicola were analysed in near-isogroups derived from the F, generation of suscep tible parent crossed with resistant parent. The results indicated that the effects of the pathogen on the activities in resistant and sus ceptible plants were different. Although the activities in both resistant and susceptible plants had a course of rising after lowing, the activities in susceptible plants rose up earlier than that in resistant plants. The differences of the zymograms mainly behaved in the score of pI 4.2~5.3, suggesting some allelic genes of peroxidases can be induced after inoculation with the pathogen and showing changes in multiple zymograms. The technical keys on study of the relation between resistance to disease and isoperoxidases were discussed.
【Key words】 near-isogroups of rice; bacterial leaf streakof rice; resistance. isoperoxidaes;
- 【文献出处】 农业生物技术学报 ,JOURNAL OF AGRICULTURAL BIOTECHNOLOGY , 编辑部邮箱 ,2000年01期
- 【分类号】S188
- 【被引频次】11
- 【下载频次】127