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苏云金芽孢杆菌ICP基因PCR鉴定的策略与进展
Strategies and Advances on PCR Identification for Insecticidal Crystal Protein Genes of Bacillus thuringiensis
【摘要】 探讨了PCR鉴定中模板来源、DNA聚合酶、引物的选择等三方面的策略,并从特异复合PCR鉴定、共同复合PCR鉴定、两步复合PCR鉴定、特异PCR鉴定、PCR-RFLP鉴定等五个角度对其进展作了回顾与展望。
【Abstract】 In this paper, strategies on PCR identification are discussed based on DNA samples、 primers and DNA polymerases. its advances are also reviewed in light of specitic multiplex PCR, general multiplex PCR, two-step multiplex PCR, specific PCR. and PCR-RFLP. The problems and perspectives are presented as well.
【关键词】 苏云金芽孢杆菌;
ICP基因;
PCR鉴定;
策略与进展;
【Key words】 Bacillus thuringiensis; ICP genes; PCR identification; strategies and advances;
【Key words】 Bacillus thuringiensis; ICP genes; PCR identification; strategies and advances;
【基金】 科技部“九五”攻关课题!96-C01-02-01;国家计委!85-504-21-01
- 【文献出处】 农业生物技术学报 ,JOURNAL OF AGRICULTURAL BIOTECHNOLOGY , 编辑部邮箱 ,2000年01期
- 【分类号】S188
- 【被引频次】36
- 【下载频次】226