【摘要】 目的 :了解早、晚期增生性瘢痕组织内 β1 、α1 整合素粘附分子表达水平及在瘢痕增生发展中的作用和意义。方法 :制备早、晚期增生性瘢痕组织石蜡切片标本 ,借助整合素粘附分子单克隆抗体 ,通过免疫组织化学染色技术检测组织内 β1 、α1 整合素的表达情况。结果 :在早、晚期增生性瘢痕组织中 ,β1 、α1 整合素粘附分子的表达有着较为明显的差异。主要表现为在增生早期瘢痕组织内β1 、α1 整合素粘附分子表达较为广泛 ,无论是表皮基底细胞 ,还是瘢痕浅层、深层以及接近正常皮肤组织边缘的成纤维细胞均有较强的阳性信号表达。而在增生晚期的瘢痕组织中 ,β1 、α1 整合素粘附分子的表达一般局限于表皮基底细胞和部分瘢痕成纤维细胞膜上 ,同时 β1 、α1 的信号强度也比瘢痕增生早期弱。结论 :早、晚期增生性瘢痕组织内 β1 、α1 整合素粘附分子表达差异一方面反映了在瘢痕增生发展过程中组织内成纤维细胞的活性变化 ,另一方面也提示了整合素粘附分子积极参与增生性瘢痕结缔组织的构建。更多还原

【Abstract】 Objective:To investigate the expression levels of β 1 and α 1 integrins in hypertrophic scar tissues.Methods:Hypertrophic scars tissue specimens were collected.The expressive levels of β 1 and α 1 integrins in specimens were determined by immunohistochemistry stainning technique.Results:Although in all hypertrophic scars tussue specimens there were ositive signals of β 1 and α 1 integrins,the distribution and intensity of signals were obviously different.In hypertrophic scar tissues of early period,we found that epidermal cells and extensive fibroblasts expressed abundant β 1 and α 1 integrins.But in hypertrophic scar tissues of later period,only partly fibroblasts located in collagen nodules and beside normal tissue were examined to express β 1 and α 1 integrins.Conclusions:During the developing process of hypertrophic scar,expressive change of β 1 and α 1 integrins in the tissues not only reflected the active situation of fibroblasts,but also suggested integrins may play an important role in scartissue remodelling. [更多还原