【摘要】 根据特异性切割马铃薯卷叶病毒中国分离株 ( PLRV-Ch)复制酶基因负链 RNA的锤头状核酶 ,设计、合成了编码与其相应的突变核酶的 c DNA,并克隆到质粒 p GEM-4 Z中 ,经序列分析及体外转录表明得到完整的突变核酶基因重组质粒 ,从而为突变核酶的转基因及进一步研究核酶在转基因马铃薯中表达引起的抗性及机理创造了条件更多还原

【Abstract】 A mutated ribozyme gene cDNA was designed and synthesized according the hammer head structure ribozyme cleaving negative strand RNA of replicase gene of potato leafroll virus chinese isolate (PLRV Ch). The mutated ribozyme gene was cloned in plasmid pGEM 4Z named pMGR, and analyzed by sequencing. Transcription in vitro experiment revealed a mutated ribozyme RNA was obtained.更多还原