【摘要】 从免疫毒理学角度研究了氯化三丁基锡 ( TBTCl)、氯化二丁基锡 ( DBTCl)和氯化一丁基锡 ( MBTCl)对小鼠免疫系统的影响。采用外周血液淋巴细胞酸性 α-醋酸萘脂酶 ( ANAE)检测法和溶血分光光度测定法 ,对小鼠腹腔注射 0 .0 1～ 0 .1 0 μg/kg TBTCl和 0 .0 1～ 0 .1 3 μg/kg MBTCl可增加 T-淋巴细胞数量和诱导抗体的产生 .而高于此剂量范围作用 7d,各指标则呈现不同程度的下降趋势 ,0 .0 1 μg/kg DBTCl对小鼠免疫系统无明显影响 ,而0 .0 5～ 0 .0 2 μg/kg DBTCl对小鼠 T和 B淋巴细胞产生不同程度的抑制作用 .更多还原

【Abstract】 From immunotoxicological point of view, the effect of tributyltin chloride (TBTCl), dibutyltin chloride (DBTCl) and monobutyltin chloride (MBTCl) were studied. We conducted different doses ranging from 0 01～0 20μg/kg TBTCl,DBTCl and MBTCl intrapertoneally (i.p.) to investigate the influence of these compounds on peripheral blood T and B lymphocytes in mice by using both peripheral blood lymphocyte acid α naphthyl acetate esterase (ANAE) detection and hemolytic colorimetric methods. The result demonstrated that dosed with 0 01～0 10μg/kg TBTCl and 0 01～0 13μg/kg MBTCl for 7 days resulted in increasing T lymphocyte in number and antibody production, while injected with 0 13～0 20μg/kg TBTCl and 0 15～0 20μg/kg MBTCl for 7 days decreased the above cited parameters. Suggested that TBTCl and MBTCl enhanced or suppressed immune system function at different dose ranges. DBTCl has shown a suppression effect on this system. Suggested that immune system is sensitive to DBTC1. The present study provided and created a new possibility for dinical immunologic disease diagnostic method, but the mechanisms by which TBTCl, DBTCl and MBTCl act on immune system should be futher more studied.更多还原