【摘要】 目的　观察乙醇对伏核神经元电活动的影响 ,探讨乙醇的中枢作用机制及与内源性阿片肽的关系。方法　制备大鼠伏核脑片 ,采用细胞外记录。结果　 (1)单独灌流乙醇 (0 .2μmol/ L )使神经元放电频率先增加后降低 ,并可被 Nal(0 .2μm ol/ L )翻转。 (2 )联合灌流阿片受体特异性激动剂 DAGO、U - 5 0、D- PEN(0 .5μmol/ L )和乙醇 ,神经元抑制程度增强 ,以 DA-GO最为显著。结论　乙醇抑制伏核神经元电活动 ,该效应与内源性阿片肽系统特别是μ受体有关。更多还原

【Abstract】 Objective The effects of ethanol (0.2 μmol/L) on the spontaneous electrical activity in the neurons of slice preparation of the nucleus accumbens septi (NAcc) was investigated. Methods Use extracellular recording.[WT5,5”HZ]Results\ [WT5,5”BZ] (1) In response to the application of ethanol into the superfusate for 10~15 min, spontaneous discharge rate (SDR) of 25 neurons was increased significantly, then, it was decreased ( P <0.01). This decreasing responses could be reversed by 0.2 μmol/L opiate antagonist naloxone (Nal), which suggests ethanol may exert some effects through interactions with endogenous opiate peptide systems of their receptors. (2) Following the application of DAGO, U 50 and D PEN for 5~10 min respectively, the effect of ethanol might be reinforced. By statistical analysis of ANOVA, we conclude that the effect of DAGO, the selective μ opiate receptor agonist, is stronger than other two opiate receptor agonists. [WT5,5”HZ]Conclusion (1) The spontaneous electrical activity of the NAcc neurons can be inhibited by ethanol. The effect of ethanol will be involved in endogenous opiate system. (2) It is possible that μ opiate receptor has the strongest effect on the ethanol inhibitory action.更多还原