【摘要】 10 0羽雏鸡笼养于 2 0℃ ,相对湿度为 75 %的环境中 ,然后将试验组鸡 (5 0羽 )转移至 40℃ ,相对湿度为 90 %的环境中 ,观察热应激对雏鸡自然杀伤 (NK)细胞活性和T淋巴细胞转化率的影响。结果表明 ,在应激 2h内 ,T淋巴细胞转化率显著降低 (P <0 0 5 ) ,而NK细胞活性则显著升高 (P <0 0 5 )。耐过鸡的T淋巴细胞转化率在 6～ 8h后基本恢复正常 ,NK细胞活性也接近正常水平。对应激鸡的胸腺、法氏囊、脾脏的病理组织学动态观察发现 ,应激持续 6～ 8h后 ,均出现出血、萎缩、颗粒变性、水泡变性、脂肪变性为特征的变化。更多还原

【Abstract】 Fifty of 100 chickens were conveyed to new condition,temperature from 20 ℃ to 40 ℃,relative humidity from 75% to 90%.The influences of heat shock on natural killer(NK)cell activities and lymphocyte proliferation rates of chickens were studied by using of LDH short release assay and MTT method respectively.The results showed that lymphocyte proliferation rates decreased significantly(P<0 05)in two hours after heat shock,while NK cell activities increased significantly (P<0 05).Lymphocyte proliferation rates and NK cell activities of endured chickens recovered to normal level on the whole during 6?8 hours after heat shock.Histopathological changes in thymus,bursa of fabricius,spleen were also observed kinetically.Stressed cells within 6?8 hours were characterized by hemorrhage,atrophy,granular degeneration,vacuolar degeneration and fatty degeneration.更多还原