【摘要】 选取了工业废水中普遍存在的几种氯代芳烃 ,以腹腔注射的方法对鲫鱼进行染毒 .分别用放射免疫测定方法测定未染毒和染毒的鲫鱼体内的性激素 (睾酮和 17β -雌二醇 )含量 ,并对测定结果进行比较 .结果发现染毒鲫鱼体内睾酮的浓度水平与未染毒鲫鱼体内睾酮水平相比存在着明显的差异 ,而 17β -雌二醇浓度水平与未染毒鲫鱼体内 17β -雌二醇水平相比基本上没有显著差异 .表明上述氯代芳烃类化合物进入水生动物尤其是高等水生动物体内后 ,能显著影响睾酮的合成与释放及其正常生理功能 ,而对 17β -雌二醇的影响还需进一步的研究更多还原

【Abstract】 During the recent decade there has been increasing concern at the levels of industrial and agricultural contaminants in the aquatic environment and the possible effect of such pollution on both human health and the well being of animal populations.Since some of these contaminants which are called endocrine disrupters have oestrogenic activities and can mimic the role of oestrogen and disturb the reproductive function of animals and human beings,more and more efforts have been made to elucidate the potential effects of these contaminants of animals especially aquatic animals.In this study,several chloroaromatic hydrocarbons chlorobenzene,1,3 dichlorobenzene,1,4 dichlorobenzene and p chloro methylbenzene,which are generally presented in industrial effluents,were selected to be studied under laboratory conditions for their effects of plasma sex steroids (testosterone and 17β estradiol)in carp (Carassius auratus) to determine whether these chemicals may act as endocrine disrupters and cause threats to reproduction of aquatic species.The 4 chloroaromatic hydrocarbons were injected into carp at different doses separately,and after 2 weeks,carp plasma was collected,centrifuged and extracted by dichloromethane,and sex steroids levels were detected using radioimmunology assay (RIA).The results showed that in comparison with the mean concentration of testosterone which was (4?072.582±227.44) pg/mL in control groups,the concentrations of testosterone in chemical treated groups significantly increased ( P < 0.05 ),and in contrast with the effect on testosterone levels,nearly no significant differences exist between concentrations of 17β estradiol in chemical treated fish and the relative 17β estradiol level of (190.66±20.16)pg/mL in control groups ( P >0.05).These results suggested that although these 4 chloroaromatic hydrocarbons may have no obvious effects on the 17β estradiol level in aquatic animals such as fish,they contribute to the disturbance of testosterone levels in fish and may cause great threats to the reproduction of fish,and thus may have some adverse effect on the balance of aquatic ecosystems.更多还原