【摘要】 目的为探讨鼻腔耐受和 Wistar大鼠对 EAMG耐受的机制。方法用放射标记的 c DNA寡核苷酸作探针原位杂交计数免疫后第 7周表达 IFN-γ、IL - 4和 TGF-β m RNA的 MNC。结果 EAMG大鼠 PIL N和脾脏中 IFN-γ m RNA表达细胞数比 CFA大鼠高 (P<0 .0 5 ) ;鼻腔耐受大鼠 PIL N中 IFN-γ和 IL - 4m RNA表达细胞数比 EAMG大鼠低 ,PIL N、脾脏和胸腺中 TGF-β m RNA表达细胞数比 EAMG大鼠高 (P<0 .0 5 ) ;WF大鼠 PIL N中 IFN-γ m RNA表达细胞数比 EAMG大鼠低 ,TGF- β m RNA表达细胞数比 EAMG大鼠高 (P<0 .0 5 )。结论 IFΝ- γ表达增高与 EAMG发生有关 ,IFN- γ表达降低 TGF- β表达增高与 EAMG耐受有关 ;TGF- β表达增高在 EAMG耐受中起重要作用。更多还原

【Abstract】 ObjectiveTo explore the mechanism of immune tolerance to experimental autoimmune myasthenia gravis(EAMG) in nasally tolerized rats and Wistar rats Methods In situ hybridization with radiolabelled cDNA oligonucleotide probes was adopted to enumerate mononuclear cells(MNC)expressing mRNA for interferon γ(IFN γ),interleukin 4(IL 4) and transforming growth factor β(TGF β) on week 7 post immunization ResultsIt was shown that MNC from popliteal and inguinal lymph nodes(PILN) and spleen of EAMG rats contained higher numbers of IFN γ mRNA expressing cells compared to CFA injected rats( P <0 05) In nasally tolerized rats MNC from PILN contained lower numbers of IFN γ and IL 4 mRNA expressing cells and MNC from PILN、spleen and thymus contained higher numbers of TGF β mRNA expressing cells compared to EAMG rats( P <0 05) In WF rats MNC from PILN contained lower numbers of IFN γ mRNA expressing cells and higher numbers of TGF β mRNA expressing cells compared to EAMG rats( P <0 05) ConclusionsHigher numbers of IFN γ mRNA expressing cells related to the development of EAMG,lower numbers of IFN γ mRNA expressing cells and higher numbers of TGF β mRNA expressing cells related to tolerance of EAMG,and that higher numbers of TGF β mRNA expres sing cells play an important role in tolerance to EAMG [更多还原