【摘要】 目的克隆人白介素 - 18结合蛋白 (h IL- 18BP)的 c DNA,以研究其结构与功能的关系。方法从中国汉族人的胎盘组织中提取总 RNA,以 RT- PCR方法扩增出全长 5 85个 bp的 IL- 18BP c DNA,将其与表达载体 pc DNA3连接 ,转化大肠杆菌 DH5 α,建立了 h IL- 18BP的 c DNA克隆。结果序列分析表明 ,与国外文献报道的人 IL- 18BP的 c DNA序列完全一致。结论 h IL- 18BP已成功地得到克隆。更多还原

【Abstract】 ObjectiveTo obtain the cDNA of human interleukin 18(IL 18) binding protein(hIL 18BP) and to investigate the relationship between structure and function of hIL 18BP.MethodsThe total RNA was extracted from human placenta and the intact cDNA of hIL 18BP was amplified by RT PCR.The cDNA was cloned into pcDNA3 vector and transformed into E.coli DH5α.Results The result of sequencing was completely consistent with the published hIL 18BP cDNA.Conclusion hIL 18BP cDNA has been cloned successfully. [更多还原