【摘要】 目的将 CD2 0胞外区基因在大肠杆菌中表达 ,并研究表达产物作为抗原筛选抗体的可能性。方法设计引物从 p U C19/ CD2 0质粒上钓取 CD2 0胞外区基因 ,克隆到 p Ex Sec 载体上 ,在大肠杆菌中融合表达。结果表达产物的相对分子质量 ( Mr)约为 2 6 0 0 0 ,Western- blot鉴定能够为抗 CD2 0分子的 m Ab所识别。利用初步纯化的 CD2 0胞外区融合蛋白为抗原 ,建立了检测 CD2 0 m Ab的 EL ISA方法。结论为研制 CD2 0分子的 m Ab奠定了基础更多还原

【Abstract】 ObjectiveTo get the antigen of CD20.MethodsWe cloned the extracellar region gene of CD20 from plasmid pUC19/CD20, and expressed it as inclusion bodies in pExSec I proyoatic systemResultsWesternblot showed that the expressed protein could specifically bind to the mAb of the CD20, and it weighted about 26kdThe purified products could be used as an antigen to screen the mAb of CD20ConclusionThis experiment lay a foundation of preparation for the mAb against human CD20. [更多还原