【摘要】 在研究应用RAPD技术对矮臀鳃金龟、华北大黑鳃金龟的文登种群、南京种群及东北大黑鳃金龟的分类地位时 ,采用 5、1 0、1 5、2 0、30和 36条引物的扩增谱带进行聚类分析。结果表明 ,在进行种间分类时 ,1 0条引物 ( 1 0 0条左右谱带 )便可得到稳定的树状图 ;在进行种下各阶元分类地位研究时 ,2 0条引物 ( 2 0 0条左右谱带 )即可。分别采用Nei的遗传距离 (D值 )和距离指数 (P值 )两种不同算法建立距离矩阵 ,研究结果表明 ,当引物数量足够多时 ,两种算法会得到相同的结果 ;但当引物数量较少时 ,遗传距离要优于距离指数更多还原

【Abstract】 Author studied the clusters result with 5,10,15,20,30 and 36 primers on the phylogeny of four species and strains: Holotrichia. ernesti, H. oblita Nanjing population and Wendeng population,H. diomphalia with RAPD. The result shown that 10 primers (100 distinct fragments) may be usefulness between species (D is greater than 0.5), 20 primers (200 distinct fragments) in subspecies and populations. The analysis were basal on genetic distance (D) and 1-similarity (P).The cluster trees shown that it was similar result when used more primers with the genetic distance or 1-similarity;the genetic distance was better than the 1-similarity when the primers used was less.更多还原