【摘要】 应用酶联免疫吸附法 (ELISA)测定了空苞栗子房和正常栗子房胚胎发育期的内源激素含量 ,并分析了BR、GA3、PP333对板栗子房和顶芽内源激素平衡的影响。结果表明 ,正常栗胚胎发育期子房内源GA1+3、iPAs 含量和 (GA1+3 +iPAs) /ABA值呈现出“双峰”型变化模式 ;而子房内源ABA含量变化趋势相反 ,且幅度较小。空苞栗子房内源激素含量及其比值在整个胚胎发育期未出现明显的峰值 ,且子房内源GA1+3、iPAs含量和 (GA1+3+iPAs) /ABA值呈下降趋势 ,ABA含量呈上升趋势。空苞栗子房内源激素GA1+3、iPAs 含量和 (GA1+3 +iPAs) /ABA值在授粉期的低水平与授粉不良有关 ,而幼胚发育期的持续下降可能导致了子房营养物质供应不足及幼胚发育停滞。BR( ρ=0 .0 1mg·L- 1)喷布提高了子房和顶芽中的GA1+3、iPAs、ABA含量及 (GA1+3、iPAs) /ABA值 ,表现出对子房发育的促进作用 ;GA3( ρ =30 0mg·L- 1)喷布使子房和顶芽中内源GA1+3 含量大幅度提高 ,ABA含量显著降低 ;PP333( ρ =10 0 0mg·L- 1)喷布显著降低了子房和顶芽中的GA1+3 含量 ,对促进板栗的胚胎发育极为有利更多还原

【Abstract】 The contents of endogenous hormones in empty shell chestnut ovary and normal chestnut ovary during the period of embryo development were determined,and the effects of BR,GA 3,PP 333 on the endogenous hormone levels in ovary and top shoot of Chinese chestnut were analyzed by means of enzyme linked immunosorbent assay (ELISA). The results showed that,the contents of endogenous GA 1+3 ,iPA s and the ratio of (GA 1+3 +iPA s)/ABA in normal chestnut ovary showed as a “double peaks” model,while the content of endogenous ABA in ovary tended to reversely change by a small range. In contrast,the endogenous hormone contents and their ratio in empty shell chestnut ovary showed no obvious peak,the contents of endogenous GA 1+3 ,iPA s and the ratio of (GA 1+3 +iPA s)/ABA appeared a descending trend while the endogenous ABA content appeared an ascending trend during whole embryo developmental period. The lower levels of endogenous GA 1+3 ,iPA s and the ratio of (GA 1+3 +iPA s)/ABA during the pollination period were related with poor pollination,and the decreases of those during the period of young embryo development could cause a serious lack of nutritious substances in ovary and a cease of young embryo development. Foliar spraying of BR (0.01 mg·L -1 ) could increase the levels of endogenous GA 1+3 ,iPA s,ABA and the ratio of (GA 1+3 +iPA s)/ABA in ovary and top shoot respectively,which showed a positive effect on embryo development. A considerable increase of endogenous GA 1+3 level and a sharp decrease of endogenous ABA level in ovary and top shoot commenced with foliar spraying of exogenous GA 3 (300 mg·L -1 ). Foliar spraying of PP 333 (1 000 mg·L -1 ) could obviously decline the content of endogenous GA 1+3 in ovary and top shoot and increase the content of endogenous ABA in top shoot,which could be of benefit to embryo development of Chinese chestnut.更多还原