【摘要】 在用蛋白质酸性水解制备氨基酸时，因中和残留的酸会使水解液中带入大量的盐。在进一步用离子交换色谱法分离混合氨基酸时，首先需脱掉其中的盐。本文用苯乙烯系强酸性阳离子交换树脂的盐型柱，根据氨基酸与苯乙烯系强酸性阳离子交换树脂之间既存在离子间的静电作用，又存在疏水作用，且二者之间存在协同作用，而盐在盐型苯乙烯系强酸性阳离子交换树脂柱上不保留的原理，用水作为洗脱剂，使盐和氨基酸（配制的盐和氨基酸混合液及含盐的毛发水解液）得到分离。本方法脱除氨基酸中的盐简单易行，用水作为洗脱剂既廉价由不造成污染，盐型树脂不用再生即可用于下次运行。研究了各种条件对分离性能的影响。更多还原

【Abstract】 In the preparation of amino acids through acidic hydrolysis of proteins, high concentration of salt is introduced into the hydrolysate when the residual acid is neutralized. The salts need to be removed if the amino acid mixture is separated further through ion exchange chromatography. In this paper, chromatographic column packed with salt-type polystyrene-based strongly acidic cation exchange resin was used to desalt the mixture of salt and amino acids (prepared by mixing or hair hydrolysate). The separation mechanism is that there are both electrostatic interaction and hydrophobic interaction between the amino acids and the resin, and there is cooperation between the two interactions. While salt does not retain on the salt-type polystyrene-based strongly acidic cation exchange resin column. Therefore the amino acids and salt can be separated on the column using water as eluant. This method is very simple and economic, because water was used as eluant, which is cheap and does not introduce pollution, and regeneration of the used salt-type resin was not needed for the next run. The effect of various conditions on the separation is studied.更多还原