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固相萃取-反相HPLC分析色酒中有机酸
rHPLC DETERMINATION OF ORGANIC ACIDS IN WINE BY SOLID PHASE EXTRACTION
【摘要】 以高效液相色谱法对三种有色酒中的七种有机酸进行了分析。采用 C18预处理柱进行样品前处理 ,固定相为 μBondapak C18柱 ,流动相为 p H2 .9的 5g· L-1磷酸氢二铵溶液 ,流速为1 ml· min-1,检测波长为 2 1 4nm,方法回收率为 96%~ 1 1 3% ,检出限为 2 0~ 1 2 5ng
【Abstract】 The rHPLC determination of seven organic acids in wine is reported in this paper, PT C 18 cartridge was used in the sample preparation. Recoveries and detection limits for these organic acids are found to be in the range of 96%~113% and 20~125ng respectively. μBondapak C 18 column is used as the stationary phase and a solution of (NH 4) 2HPO 4 (5g·L -1 , pH 2.9) is used as the mobile phase. A flow rate of 1ml·min -1 is employed in the determination and the measurements are made at the wavelength 214nm.
【关键词】 高效液相色谱法;
固相萃取;
有机酸;
色酒;
【Key words】 rHPLC determination Solid phase extraction Organic acids Wine;
【Key words】 rHPLC determination Solid phase extraction Organic acids Wine;
- 【文献出处】 理化检验(化学分册) ,Physical Testing and Chemical Analysis Partb Chemical Analgsis , 编辑部邮箱 ,2000年04期
- 【分类号】O657.7
- 【被引频次】26
- 【下载频次】389