【摘要】 目的 :了解异丙酚对大鼠脑一氧化氮 (NO) /环鸟苷酸 (cGMP)信号转导系统的影响。方法 :32只SD大鼠 ,随机分为对照组和异丙酚组 ,分别腹腔注射 (ip)生理盐水 10ml/kg或异丙酚 10 0mg/kg。用分光光度法测定脑组织一氧化氮合酶 (NOS)活性和NO产量 ,放射免疫法测定脑组织cGMP含量。结果 :与对照组比较 ,大鼠ip异丙酚 10 0mg/kg不但明显抑制小脑、海马和大脑皮层的NOS活性 ,而且显著减少上述脑区NO产量和cGMP含量 (P <0 0 5或P <0 0 1)。结论 :NO/cGMP信号转导系统可能在异丙酚的全麻分子学机理中发挥重要作用更多还原

【Abstract】 Objective: To investigate the effects of propofol on NO/cGMP signal pathway in rat brain.Methods:32 SD rats were divided randomly into control group and propofol group and either normal saline 10ml/kg or propofol 100mg/kg was administered introperitoneally(ip).Nitric oxide synthase(NOS) activity and NO production were assessed by spectrophotometric analysis.cGMP content was measured by radioimmunoassay.Results:Propofol 100mg/kg significantly inhibited NOS activity and reduced NO production and cGMP content of the cerebellum,hippocampus and cerebral cortex as compared with those of control group (P<005 or P<001).Conclusion:NO/cGMP signal pathway may play an important role in the molecular mechanism of propofol anesthesia.更多还原