【摘要】 目的　研究中间普里沃菌在牙周炎病人病变部位和健康部位龈下菌斑中的分布。方法　选择 6 4例成年人牙周炎患者 ,分别取病变部位和健康部位龈下菌斑 ,经厌氧培养 ,挑取产黑色素菌落 ,经多聚酶链反应鉴定中间普里沃菌。结果　共对 6 14株产黑色素 G- 厌氧杆菌进行多聚酶链反应鉴定 ,有 112株被鉴定为中间普里沃菌 ;在病变部位和健康部位 ,中间普里沃菌的检出率分别是 2 8.1%和 12 .5 % ,有统计学差异 (P<0 .0 5 ) ;在病变部位和健康部位 ,中间普里沃菌的检出株数分别是 79和 33,占产黑色素 G-厌氧杆菌分离数的 2 1%和 13.9% ,有统计学差异(P<0 .0 5 )。结论　中间普里沃菌是可疑的牙周致病菌。更多还原

【Abstract】 WT5”BZ]Objective To determine the distribution of Prevotella intermedia (P.i) in subgingival plaques from diseased and healthy sites of patients with adult periodontitis (AP). Methods Subgingival plaques were collected from 64 AP patients and cultivated under anaerobic conditions. Black pigmented colonies were isolated, from which P.i strains were identified with Polymerase Chain Reaction assay. Results 614 straings of black pigmented anaerobic rods were isolated and 112 strains were identified as P.i among them. P.i was found to be 28.1% and 12.5% positive in diseased and healthy sites respectively with statistic significance ( P <0.005), 79 strains were from diseased sites and 33 strains from healthy sites which constituted 21% and 13.9% of the total blackpigmented anaerobic rods respectively. Conclusion The study indicates that P.i is a suspected pathogen of periodontitis. [WT5”HZ]更多还原