【摘要】 目的：揭示中国人ＩＤＤＭ的遗传分子基础。方法：应用分子生物学研究方法，对ＨＬＡ－ＤＲＢ进行序列特异性引物的ＰＣＲ扩增，鉴定了８１例ＩＤＤＭ患者和８４例正常对照汉族人群的ＤＲＢ基因多态性。结果： ＩＤＤＭ患者中ＤＲＢ１＊０３，ＤＲＢ１＊０９等位基因频率明显高于对照组，其频率分别为 ８．６４％ Ｖ．Ｓ ３．０％和２８．４％ Ｖ．Ｓ １６．１％（Ｐ＜０．０５）； ＩＤＤＭ患者中ＤＲＢ１－ＤＲＢ３／ＤＲＢ１－ＤＲＢ４基因型频率明显高于对照组，其频率为 １９．８％ Ｖ．Ｓ ６．７％（Ｐ＜０．０５）；证明 ＤＲＢ１＊１２等位基因与中国人 ＩＤＤＭ呈正相关。结论： ＤＲＢ１＊０３和ＤＲＢ１＊０９是中国人ＩＤＤＭ的易感基因； ＤＲＢ１＊１２是其次易感基因； ＤＲＢ１＊１５和ＤＲＢＩ＊１６是其抗性基因。更多还原

【Abstract】 Objection:To indicate the genetic molecular basis in Chinese.Method: We Applied molecular biological technique to do the specula amplification on HLA-DRB using PCR. and identified the Dan gene polymorphism in 81 patients with IDDM and that of 84 normal control from Han Nationality. Results: The frequency. of DRB1*03,DRB1*09 in patients with IDDM was obviously higher than that of controls. The frequency was 8.64%V.S 3.0% and 28.4% V.S 16. 1% respectively (P<0.05),The frequency of DRB1- DRB3/DRB1-DRB4 in patients was obviously higher than of controls, 19.8% V.S 6.7%(P<0.05), We identified the that there was positive correlation between DRB1*12 allele and IDDM in Chinese.Conclusion: DRB1*03 and DRB1*09 are the susceptible genes Chinese; DRB1 * 12 is the inferior susceptible gene; DRB1* 15 and DRB1*16are the resist genes.更多还原