【摘要】 应用免疫组化LSAB法 ,比较二磷酸核苷激酶 (NDPK)在云锡矿工肺癌和富源农民肺癌中的表达 ,以阐明nm2 3蛋白的作用 .结果 :NDPK在云锡矿工和富源农民肺鳞癌中表达阳性率分别为 56 4 %( 2 2 /39)和 58 3% ( 7/12 ) (P >0 0 5,列联系数 0 112 1) ;肺腺癌中表达阳性率为 61 1% ( 11/18)和 84 2 %( 16/19) (P <0 0 5,列联系数 0 4 4 14) .在云锡矿工和富源农民肺癌无淋巴结转移组表达阳性率分别为66 6% ( 2 8/42 )和 78 2 ( 18/2 3) (P >0 0 5,列联系数 0 2 195) ;在富源农民肺癌有淋巴结转移组 ( 62 5% ,5/8)表达阳性率高于云锡矿工肺癌有淋巴结转移组 ( 33 3% ,5/15) ,且差异有显著意义 (P <0 0 5,列联系数 0 4 70 2 ) .云锡矿工肺鳞癌无淋巴结转移者表达阳性率为 62 0 % ( 18/2 9) ,有淋巴结转移者为 4 0 0 %( 4 /10 ) ,差异有显著意义 (P <0 0 5,列联系数 0 3665) .未发现NDPK的表达水平与云锡矿工肺腺癌、富源农民肺鳞癌和肺腺癌有或无淋巴结转移有相关关系 .也未发现其与富源农民肺癌临床病理分期和分化程度有相关关系 .结论 :NDPK参与云锡矿工肺鳞癌淋巴结转移过程的调节 ;未发现其在富源农民肺鳞癌和肺腺癌中的转移抑制作用 ;NDPK的作用可能与致瘤因素有关更多还原

【Abstract】 By means of labeled streptavidin-biotin (LSAB), the expression of nm23 protein in Yunnan Tin Miners lung cancer( A group) was compared with Fuyuan peasants lung cancer ( B group) to clarify the function of nm23 protein. Results: The postive staining rate in squamous cell carcinoma of A group and B group was 56 4%(22/39) and 58 3%(7/12) respectively. There was no significant difference ( P >0 05, contingency coefficient was 0 112 1). The expression was higher in adenocarcinoma of B group (84 2%, 16/19) than in adenocarcinoma of A group (61 6%, 11/18). There was significant difference ( P <0 05, contingency coefficient was 0 441 4). Of 42 patients without lymph node metastasis in A group, 28(66 6%) showed positive staining. Of 23 patients without lymph node metastasis in B group, 1(78 2%) showed positive staining. No significant difference ( P >0 05, contingency coefficient was 0 219 5) in nm23 protein was observed in both group without lymph node metastasis. The positive staining rate in A group and B group with lymph node metastasis was 33 3%(5/15) and 62 5%(5/8) respectively. There was significant difference ( P <0 05, contingency coefficient was 0 470 2). The nm23 protein expression in A group squamous cell carcinoma without lymph node metastasis (62 0%, 18/29) was significant increased compared to that in A group squamous cell carcinoma with lymph node metastasis(40 0%, 4/10)( P <0 05, contingency coefficient was 0 366 5). It was not discovered that NDPK expression levels was correlative to adenocarcinoma in A group, squamous cell carcinoma and adenocarcinoma in B group with or without lymph node metastasis. Conclusion: nm23 protein participates in the regulation of metastasis process in Yunnan Tin Miners pulmonary squamous cell carcinoma. There is no evidence of metastasis suppression effect by nm23 protein in the pulmonary squamous cell carcinoma and adenocarcinoma of Fuyuan Peasants. The function of nm23 gene may be correlated with carcinogenic substance. In both cell types of carcinoma in Fuyuan Peasants, expression of nm23 was not correlated with tumor cell differentiation, nor was it correlated with the P—TNM staging.更多还原