【摘要】 用简易切割法制作hSCI和tSCI模型 .4 5只SD大鼠随机分为 3组 :ISC组 6只 ,hSCI组 18只 ,tSCI组 2 1只 .用显微剪在T13～L1间横向完全剪断脊髓或其一侧 1/2 ,制成tSCI和hSCI模型 .术后动态行为观察和神经功能综合评分 (CBS) ,并分别于术后 2 4h ,7d和 2 1d取T11～L3节段脊髓作冰冻切片苏木精染色观察 .结果显示 ,3组间CBS两两比较经q检验均有显著性差异 (P <0 0 1) .病理改变 ,hSCI组主要是伤侧灰质缩小 ,空泡大量出现而神经元减少且明显皱缩 ,腹角为甚 ,对侧也有类似表现且下节段较上节段严重 ;tSCI组与hSCI组伤侧相似 ,但Waller’s溃变上节段较下节段严重 .上述结果与人类SCI相似 .提示本法可作为hSCI和tSCI模型 ,具有可靠性和简便实用性 .更多还原

【Abstract】 To provide simple and stable models of spinal cord injury (SCI),the simple method was utilized in 45 Srague-Daweley rats which were divided at random into intact spinal cord (ISC) group,hemisected SCI (hSCI) group and transected SCI (tSCI) group. Spinal cords were hemisected or transected between T 13 and L 1 in hSCI group or tSCI group. Combined behavioural scale (CBS) was employed for above 3 groups after operation every day,and the difference among them was analyzed by q test. Rats suffering for hSCI or tSCI were sacrificed at 24 hours,7 days and 21 days after operation. Spinal cords from T 11 to L 3 of them were removed out and sectioned into sections 20 μm in thickness for histological observation. The results showed that the difference of CBS in above 3 groups at 24 hours,7 days and 21 days after operation was significant ( P <0 01).Moreover,the decrease of areas of gray matter in hSCI group were observed being concomitant with a batch of empty cavities in gray matter in which neurons shrunk in lesion side and the same change was observed in another side but less serious than that in lesion side. Similarly,changes in tSCI group were the same as that in hSCI group except Waller’s degeneration in proximal segment being more serious than that in distal segment. The results were consistent with the characteristic of human SCI. It indicated that above models of SCI be practicable and reliable. Based on this,we thought that hSCI and tSCI can be used as the model of hSCI and tSCI.更多还原