【摘要】 为了解胰高血糖素和双丁酰环腺苷酸在肝脏载脂蛋白代谢中的作用 ,以培养的人肝癌细胞系HepG2细胞为研究对象 ,采用“冻干浓缩培养液载脂蛋白测定法” ,观察了胰高血糖素和双丁酰环腺苷酸对HepG2细胞分泌载脂蛋白AⅠ、AⅡ、CⅢ、B10 0及E的影响。结果表明 ,胰高血糖素浓度为 4× 10 -7mol/L时 ,载脂蛋白AⅠ、AⅡ、B10 0及E的分泌分别减少 47.7%、5 4.1%、32 .7%和 33.2 % (P <0 .0 1) ,载脂蛋白CⅢ的分泌增加 89.1% (P <0 .0 1) ;双丁酰环腺苷酸的浓度为 1× 10 -3mol/L时 ,载脂蛋白AⅠ、B10 0和E的分泌分别减少 12 .3 %、2 4.9%和 16 .1%(P <0 .0 1) ,载脂蛋白AⅡ和CⅢ的分泌分别增加 6 6 .1%和 142 .6 % (P <0 .0 1)。胰高血糖素和双丁酰环腺苷酸对HepG2细胞载脂蛋白分泌的影响基本一致。更多还原

【Abstract】 Aim To study the effects of glucagon, dibutyryl cAMP on secretion of apolipoproteins AⅠ,AⅡ,CⅢ,B100 and E by HepG2 cells. Methods The apolipoprotiens contents in culture media were measured by radioimmuodiffusion assay (RID) kits developed by authors’ research laboratory. 20-fold lyophilizely condensed culture media were used for the assays. Results When the concentration of glucagon is 4×10 -7 mol/L in culture media, the secretion of apoAⅠ,AⅡ,B100 and E decreased 47.7%, 54.1%, 32.7% and 33.2%(P<0.01) respectively, while the secreation of apoCⅢ increased 89.1%(P<0.01); When the concentration of dibutyryl cAMP is 1×10 -3 mol/L in culture media, the secretion of apoAⅠ,B100 and E decreased 12.3%, 24.9% and 16.1% (P<0.01)respectively, while the secretion of apoAⅡ and CⅢ increased 66.1% and 142.6%(P<0.01). Conclusion [WT5”BZ]Glucagon and dbcAMP had similar effects on the secretion of apolipoproteins by cultured HepG2 cells.更多还原