【摘要】 为了研究卵磷脂和鞘磷脂对高密度脂蛋白 3介导大鼠腹腔巨噬细胞内胆固醇流出的影响 ,测定了细胞内胆固醇含量、磷脂含量及游离胆固醇 -胆固醇酯平衡的变化。结果发现 ,各组细胞内胆固醇含量从 79.75 μg/皿变为 42 .79、37.16、31.5 3μg/皿 ;高密度脂蛋白 3组和卵磷脂组中卵磷脂磷含量分别为 13.95和 16 .2 8μg/皿 ;高密度脂蛋白和鞘磷脂组中鞘磷脂磷含量分别为 5 .0 3和 7.0 1μg 皿。细胞与BSA、卵磷脂、鞘磷脂单独孵育后 ,细胞内胆固醇含量分别为 75 .30、74.6 5和 73.0 9μg/皿 ,游离胆固醇 /总胆固醇比值分别为 5 9.42 %、5 6 .95 %和 6 4.77%。结果提示卵磷脂和鞘磷脂均能促进高密度脂蛋白 3介导细胞内胆固醇流出 ,后者强于前者 ,而卵磷脂和鞘磷脂单独不能促进细胞内胆固醇的流出。鞘磷脂促进细胞内胆固醇酯向游离胆固醇转化 ,卵磷脂促进细胞内游离胆固醇向胆固醇酯转化更多还原

【Abstract】 Aim To study the effect of phosphalipids on the cholesterol efflux from rat macrophages mediated by HDL 3. Methods Measure the cholesterol content, cellular phospholipid content, and the balance between free cholesterol and cholesteryl ester when phosphatidylcholine (PC) or sphingomyelin (SPM) was added to the mediun. Results The cholesterol content changed from 79.95 μg/plate to 42.79, 37.16 and 31.53 in HDL 3, HDL 3+PC and HDL 3+SPM group respectively. The cellular phosphorous content in PC (PC-p) were 13.95 and 16.28 μg/plate in HDL 3 and HDL 3+PC group respectively. The cellular phosphorous content in SPM (SPM-p) were 5.03 and 7.01 μg/plate in HDL 3 and HDL 3+SPM group respectively. After macrophages were incubated with BSA, PC and SPM alone, the cellular cholesterol content were 75.30, 74.65 and 73.09μg/plate respectively and the ratio of free cholesterol to total cholesterol were 59.42%, 56.95% and 64.77% respectively. Conclusion PC and SPM couldn’t mediate directly cellular cholesterol efflux, but they could enhance cellular cholesterol efflux from macrophages mediated by HDL 3, and the ability of SPM was stronger than PC. SPM could induce cholesteryl ester convert into free cholesterol. In macrophages and PC could induce free cholesterol convert into cholesteryl ester.更多还原