【摘要】 采用中华蜜蜂ApisceranaceranaF .临床自然发病的欧洲幼虫腐臭病 (EuropeanFoulbrood ,EFB)幼虫 ,对其病原体进行了分离鉴定。结果表明 :中华蜜蜂EFB的病原系蜂房蜜蜂球菌Melissococcuspluton。该菌为革兰氏阳性的兼性厌氧菌 ,形态学及染色特性、致病性试验、血清学试验和细菌DNAG +Cmol%试验均证明中华蜜蜂和西方蜜蜂的EFB病原属于同属的蜂房蜜蜂球菌。生理生化特性结果与国外的早期研究结果相近似。该试验为不同地理位置、不同种寄主间蜂房蜜蜂球菌的遗传差异的研究 ,以及中华蜜蜂EFB病的防治学研究打下基础更多还原

【Abstract】 The pathogen of the Apis cerana cerana F. larvae naturally infected with European Foulbrood (EFB) was isolated and identified. The results indicate that the pathogen is Melissococcus pluton, a Gram positive anaerobe or microaerophile. Its cellular morphology, stained characteristics, infecfivity, serology and G+C content in DNA are similar to that in A.mellifera L.,suggesting the EFB pathogen of A.cerana cerana F.to be Melissococcus pluton as in A.mellifera. The physiological and biochemical characteristics of EFB pathogen in Apis cerana cerana F.are approximately in accordance with an earlier study on A.mellifera L.by M.F.Allen.更多还原