【摘要】 利用聚丙烯酰胺梯度凝胶电泳检测了棉铃虫Helicoverpaarmigera的 13种等位酶 :α 磷酸甘油脱氢酶 (α GPDH)、酸性磷酸酯酶 (ACPH)、碱性磷酸酯酶 (ALP)、醛氧化酶 (AO)、酯酶(EST)、谷氨酸草酰乙酸转氨酶 (GOT)、己糖激酶 (HEX)、亮氨酸氨肽酶 (LAP)、乳酸脱氢酶(LDH)、苹果酸脱氢酶 (MDH)、苹果酸酶 (ME)、磷酸葡萄糖变位酶 (PGM )和黄嘌呤脱氢酶(XDH) ,染色采用双染法。对其中 9种等位酶的遗传变异进行了分析 ,包括 13个位点 ,6个位点表现出多态性 ,7个位点是单态的 ,其中多态性位点比例为 46 15 %。AO、GOT、LAP、LDH、ME和XDH计算出棉铃虫的平均杂合度为 0 116 0 ,南京、成都、武穴、衡阳和哈密 5个种群的平均遗传距离为 0 0 0 0 8～ 0 0 2 93,平均遗传相似度为 0 970 7～ 0 992。棉铃虫种群内存在很高的遗传多态性 ,而已测定的种群间遗传分化程度较小 ,种群间没有基因交流的障碍。迁飞阻碍了不同地理种群间的遗传分化更多还原

【Abstract】 Thirteen allozymes,α glycerophosphate dehydrogenase (α GPDH), acid phosphatase (ACPH), alkaline phosphatase (ALP), aldehyde oxidase (AO), esterase (EST), glutamate oxaloacetate transaminase (GOT), hexokinase (HEX), leucine aminopeptidase (LAP), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), malate enzyme (ME), phosphoglucomutase (PGM), xanthine dehydrogenase (XDH), were analysed by polyacrylamide gradient gel electrophoresis with double stain method. The genetic variations of 9 allozymes within and among 5 geographic populations were investigated. Among the 13 loci examined, 6 loci were polymorphic and 7 loci were monomorphic. The percentage of polymorphic loci was 46 15%. As calculated with the results of AO, GOT, LAP, LDH, ME and XDH, the average heterozygosity of Helicoverpa armigera was 0 1160. Among Nanjing, Chengdu, Wuxue , Hengyang and Hami populations, their mean genetic distance was 0 0008～0 0293 and mean genetic similarity was 0 9707～0 992. It means that there exits high genetic diversity in each population, but the genetic differentiation among these populations is very limited. There was no barrier of gene flow among these investigated populations. The migration could hamper more genetic differentiation among different geographic populations.更多还原