【摘要】 研究益气活血方药理血清对大鼠贮脂细胞增殖作用机制。通过制备 3周、5周、7周的肝纤维化模型大鼠血清、正常大鼠血清和益气活血复方药物血清 ,培养肝星形细胞HSC(Hepaticstellatecells)和肝Bel74 0 2瘤株 ,采用MTT法观察和分析HSC的增殖作用。结果表明 :①益气活血方剂血清能抑制 5周、7周模型血清培养的继代HSC的增殖 ,P <0 .0 5。②益气活血方剂血清可促进 3周模型血清培养的继代HSC和模型血清培养的原代HSC的增殖 ,P <0 .0 5。③益气活血方剂血清和3周模型血清共同作用促进培养的Bel74 0 2的增殖 ,P <0 .0 5。提示益气活血方剂能够对HSC和Bel74 0 2细胞的增殖产生影响。HSC的生活状态 ,即HSC发育时相是决定益气活血方剂具有抑制或促进增殖作用的关键事件更多还原

【Abstract】 Object: To study the hepatic stellate cell(HSC) proliferation in DMEM which contained rat sera with the recipe of YiQiHuoXue. Methods: Rat sera were taken respectively from normal rats, rats with recipe of YiQiHuoXue and rats model under liver fibrosis condition for 3, 5 and 7 weeks. Proliferating effect of HSC and Bel7402 cells in vitro were analyzed through MTT method. Results: 1. The sera with YiQiHuoXue can inhibit the proliferation of subculture HSC, which was cultured in model sera for 5 and 7 weeks, P <0.05. 2.The sera with YiQiHuoXue can promote the proliferation of subclture HSC which was cultured in model sera for 3 weeks and the proliferation of primary HSC which was cultured in model sera for 3,5 and 7 weeks, P <0 05.3.The sera with YiQiHuoXue can promote the proliferation of Bel7402 cell, which was cultured in model sera for 3 weeks, P <0.05.Conclusion: The recipe of YiQiHuoXue could infect the proliferation of HSC and Bel7402. HSC proliferation depends on whether HSC was inhibited or promoted.更多还原