【摘要】 用对苯二酚和甲醛在酸催化下制得了新一类凝胶 (称 PF凝胶 ) ,可作为载体用于多种酶及蛋白质的固定。对糖化型淀粉酶的固定 ,使淀粉转化为葡萄糖 ,产率高达 97%以上 ,而且可以装入反应器连续使用 ,在常温常压下连续反应 68小时后 ,其产率仍保持不变 ,可见此固定化酶具有优良的稳定性。更多还原

【Abstract】 The PF gel type resin has been synthesized by polycondensation of hydroquinone with formaldehyde in the presence.of acidic solution. It is effective carrier for immobilization of various enzymes and proteins. Under optimum conditions,lg of this carrir(water sorption ca 90%)was placed in 10ml HAC NaAc buffer (pH4.6) of glucoamylase and suspension was shaked for 4h at15℃.The suspension was filtered and washed with excess HAC NaAc buffer. Activity ofimmobilized glucoamylase was determined,it is ca 39000u/g (dry).Immobilized glucoamylase can be used repeatedly and continuously for conversion of corn starch, liquefaction through α amylase to glucose.After continuously using 68hr,yield of glucose still reaches over 97%.更多还原