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中空纤维超滤膜在青霉素酰化酶浓缩中的应用
Application of Hollow Fiber Ultrafiltration Membrane for Condensation of Penicillinacylase
【摘要】 介绍了用超滤代替硫铵沉淀浓缩青霉素酰化酶的方法 ,不仅可缩短浓缩时间 ,而且能有效地去除其中小分子杂质。研究了膜的截留分子量、操作条件和温度对酶的活力的影响
【Abstract】 This paper introduces the technique of condensed penicillinacylase with ultrafiltration instead of precipitated ammonium sulfate. It was found that using ultrafiltrated membrane not only would save the concentrated time, but also could effectively remove iow molecular impurities.The influence of blocked molecular weight of membrane, operating conditions and temperature on the penicillinacylase activity was studied.
【关键词】 中空纤维;
超滤;
青霉素酰化酶;
浓缩;
【Key words】 hollow fiber; ultrafiltration; penicillinacylase; condensation;
【Key words】 hollow fiber; ultrafiltration; penicillinacylase; condensation;
- 【文献出处】 江苏石油化工学院学报 ,JOURNAL OF JIANGSU INSTITUTE OF PETROCHEMICAL TECHNOLOGY , 编辑部邮箱 ,2000年03期
- 【分类号】TQ02
- 【被引频次】11
- 【下载频次】173