【摘要】 目的 :探讨切应力对内皮细胞膜流动性的影响。方法 :选择 15dyne/cm2 、50 dyne/cm2 、30 0 dyne/cm2 三种切应力 ,分别模拟大中动脉处、大中动脉分叉处及病理状态下的切应力 ,以体外培养的人脐静脉内皮细胞作为实验对象 ,分别测定三组切应力作用 0 h、1h、2 h、4 h、6 h、10 h的内皮细胞膜流动性。结果 :切应力可降低内皮细胞膜流动性 ,且切应力越大 ,作用越强。切应力作用 4 h,内皮细胞膜流动性下降至最低点 ;此后随着时间延长 ,内皮细胞膜流动性不再下降 ,而形成一个平台期。结论 :切应力可影响内皮细胞膜流动性 ,进而改变内皮细胞功能更多还原

【Abstract】 To study the effect of shear stress on endothelium membrane fluidity. Methods: The culture HUVECs were treated with 3 kinds of shear stress: 15, 50, 300 dyne/cm 2 (imitating the shear stresses of the middle big artery, the bifurcation of the middle big artery, the pathological place of the artery). The membrane fluidity of the 3 groups was detected at 0,1,2,4,6,10 h. Results: The shear stress decreased the membrane fluidity. The higher shear stress, the lower the membrane fluidity. It arrived at the lowest point in 4 h, then kept at this level. Conclusion: The shear stress affects the membrane fluidity apparently, it changes the function of EC, take part in the production and development of atherosclerosis.更多还原