【摘要】 目的　检测 SA- 30 (sperm antigen- 30 )抗原在小鼠精子发生、成熟、获能及受精后的分布变化。　方法　免疫组织化学 L SAB法。　结果　 SA - 30在小鼠睾丸内的各级生精上皮均有分布 ,尤其是靠近管腔的精子细胞染色最深 ;附睾各段的上皮细胞也有 SA - 30存在 ,而且主要集中在核上区 ;在附睾头和附睾尾的精子 ,SA - 30主要分布在顶体区 ,两者无明显区别 ,获能后的精子除在顶体区有阳性染色外 ,尾部和顶体后区也出现了阳性反应。妊娠 d3的小鼠胚泡表面有 SA- 30存在 ,而妊娠 d6的胚胎上则未检测到 SA- 30。　结论　 SA- 30出现在小鼠精子发生的早期 ,附睾内精子成熟过程中 ,其表面 SA - 30的分布没有明显变化 ,而且在获能后仍然存在 ,早期小鼠胚上也有SA- 30的分布 ,从而提示 SA- 30可能参与受精过程及早期胚胎的发育。更多还原

【Abstract】 Objective\ Understanding the distribution and changes of SA\|30 during mouse spermatogenesis,maturation,capacitation and fertilization. Methods\ Immunohistochemistry was used. Results\ In testis,positive staining was found in the spermatogenic cells with a higher reactivity in the spermatids.In epididymis,the epithelia of caput,corpus and cauda epididymis were all stained,and the positive sites were mainly restricted to the superanuclear areas.On the smears of caput and cauda sperms,the acrosomal region was strongly stained,while the stainig on postacrosomal region was negative.After capacitation,however,the sperm tail and postacrosomal region were also stained.SA\|30 was also found on early embryos on day 3 of pregnancy, but not on day 6 of pregnancy. Conclusions\ SA\|30 begins to appear in the early stage of mouse spermatogenesis;SA\|30 on sperm surface does not show apparent changes during epididymal maturation and does not loss during capacitation; SA\|30 also occurs on early mouse embryos;SA\|30 may be involved in fertilization and the development of early embryos.更多还原