【摘要】 本研究用一种简便方法提取大鼠脑线粒体 DNA(m t DNA) ,所得的线粒体 DNA纯度为 1.8± 0 .0 1,得率为每克脑组织 0 .8～ 1.0 μg线粒体 DNA,以此为模板进行 PCR反应 ,扩增线粒体 DNA编码基因 ,产量丰富、特异性高。该方法操作简便、成本低 ,适用于一般实验室更多还原

【Abstract】 A simple technique for isolation of mitochondrial DNA from rat brain is described. The purity (260/280) of mtDNA extracted by the method is 1.8±0.01, the mtDNA yield per gram brain is 0.8 10/μg. The product of the PCR is specific. The method can be used in general laboratory because it costs very low and is easy to operate.更多还原