【摘要】 目的 :研究系统性红斑狼疮 (SL E)患者外周血 TH1类细胞因子基因的表达 ,探讨其在 SL E发病过程中的作用。　方法 :采用荧光标记 RT- PCR半定量的方法 ,对 32例 SL E患者和 2 5例健康对照者外周血单个核细胞(PBMC)白介素 - 2 (IL- 2 )和 γ干扰素 (IFN- γ) m RNA的表达水平进行检测。　结果 :活动性 SL E患者 IL- 2和 IFN-γ m RNA的表达为 (0 .82± 0 .2 0 )和 (0 .89± 0 .31)与正常对照组水平 (1.12± 0 .11)和 (1.2 8± 0 .15 )相比 ,可见显著降低 (P<0 .0 1)。　结论 :活动性 SL E患者 PBMC中 TH1类细胞因子的基因表达量下调 ,可能在 SL E疾病发生过程中有重要作用。更多还原

【Abstract】 Objectives:To study the gene expression of T H1 type cytokines in peripheral blood of patients with systemic lupus erythematosus(SLE) and their role in the pathogenesis of SLE. Methods:Thirty two SLE patients and 25 normal controls were chosen and the expression of IL 2 and IFN γ mRNA of peripheral blood mononuclear cells(BPMC) were analyzed by fluorescence labeled RT PCR semiquantitative method. Results:The respective levels of IL 2 mRNA and IFN γ in 32 patients with SLE were (0.82±0.20) and (0.89±0.31), which were significantly lower than that of 25 normal controls(1.12±0.11) and (1.28±0.15), P <0.01. Conclusions: The gene expression of T H1 type cytokines in the active stage of SLE is down regulated and may play an important role in the pathogenesis of SLE.更多还原