【摘要】 选用经筛选的 2 0个短序列引物 ( 1 0 nt)和随机抽取的 2个随机长序列引物 ,对激光诱导产生的无核沙田柚 (处理组 )及未经激光处理的有核沙田柚 (对照组 )进行了 RAPD(随机扩增多态性 DNA)分析。结果有6个引物扩增出了共 2 0条多态性片段 ,从而表明在沙田柚的处理组与对照组之间 ,存在着 DNA分子水平上差异 ,也就是说 ,已经引起了沙田柚后代基因组的显著变异 ,且这种诱导所引起的性状改变是可以遗传的更多还原

【Abstract】 Twenty short arbitrary primers(10nt)and two long ones(20nt) were used in random amplified polymorphic DNA(RAPD) analysis for different strains of Shatian Pumelo. Among them, six primers could generate twenty polymorphic bands. The result was shown that there were some genetic variations among the Shatian Pumelo stains irradiated by laser and nonirradiated with RAPD method. And the analysis of PAPD demonstrated that the laser irradiation can induce the genetic variation of Shatian Pumelo.更多还原