【摘要】 观察浓烟吸入所致大鼠急性肺损伤与透明质酸(Hyaluronic Acid,HA)、层粘蛋白（Laminin, LN）含量的关系。将SD大鼠随机分成正常对照组和吸烟后不同时间观察组。分别测定血清、肺组织和支气管肺泡灌洗液中HA及LN含量，支气管肺泡灌洗液中炎性细胞数及比例，并用光镜和电镜观察肺组织病变。结果显示，吸烟后血清和肺组织中HA含量升高，12h达到高峰。血清和肺组织中HA含量分别为（470.4±97.5）μg/L和（697.5±115.1）μg/L；对照组分别是（186.4±31.5）μg/L和（202.6±39.4）μg/L。支气管肺泡灌洗液HA在18h显著升高，而LN各组无明显变化。支气管肺泡灌洗液中细胞数在12h最多，主要是中性粒细胞。提示HA升高似可作为浓烟吸入急性肺损伤的病理指标。更多还原

【Abstract】 The purpose of this study was to assess the relatio nship between acute pulmonary injury induced by smkoe inhalation and levels of H A and LN. 40 rats were divided randomly into 5 groups,control group and experim ental groups of different length of time elapsed after smkoe inhalation. HA and LN in sera、bronchoalveolar lavage fluid（BALF）and lung tissue，as well as inf lammatory cells were determined; pulmonary pathgenic changes were detected by l ight and electron microscope. It was found that after smoke inhalation HA w as increased in sera and lung tissue with the peak values at 12h.The values in sera and lung tissue are 470.4±97.5μg/L and 697.5±115.1μg/L res pectively,while those of control groups are 186.4±31.5μg/L and. 202 .6±39.4μg/L respectively.HA in BALF was increased markedly at 18h， but L N of all groups showed no distinct change. Analysis of BALF showed the highest cell count at 12h，with neutral granulocytes as the main component. Increased H A might be used as an indicator of the acute pulmonary injury induced by smoke inhalation.更多还原