【摘要】 利用 6 0个随机引物对来自我国不同稻区的水稻细菌性条斑病菌株基因组DNA进行PCR扩增 ,其中2 0个引物均出现清晰和重复性的DNA扩增片段 ,扩增片段大小在 0 .5kb至 3.5kb ;应用Statistics软件UPGMA程序进行树状聚类分析 ,聚类结果表明 ,我国水稻细菌性条斑病菌具有明显的遗传分化 ,在遗传距离为 0 .30时 ,供试菌株可划分为 7个遗传相似组 (谱系 )。其中第 1、第 2和第 5组为优势组群 ,分别由 7、8、6个菌株组成。根据菌株在 12个已知基因品种上的抗感反应 ,将供试菌株分为 13个致病型 ,在遗传距离为 0 .5 0水平上聚类归属于 6个簇。菌株的致病类群与其DNA的遗传变异具有弱相关性更多还原

【Abstract】 RAPD was used to analyze the genetic diversity and virulence phenotypes of Xanthomonas oryzae pv. oryzicola , the bacterial leaf streak pathogen of rice in China. Twenty of 60 used random primers produced consistent and clear banding patterns that generated a set of products from 0.5 kb to 3.5 kb in size. Cluster analysis was conducted using UPGMA. The result showed that genetic variation existed obviously among isolates of rice bacterial leaf streak (BLS) from different regions,isolates tested could be divided into 7 lineages (groups) .Lineages 1,2 and 5 were the most frequently detected,including 7,8 and 6 strains respectively. Thirteen pathotypes(virulence phenotypes) of X.oryzae pv. oryzicola were identified according to resistant and susceptible react of 12 genotypes to the strains using 12 rice near iso lines, each containing a single gene for resistance.The 13 pathotypes grouped into six clusters. There was a weak correlation between RAPD groups and virulence phenotypes.更多还原