【摘要】 目的　检测吸入烹调油烟 (COF)的大鼠肺组织中P53 蛋白含量 ,以测试烹调油烟的毒性作用。方法　亚急性吸入浓度为 (43± 4 )mg m3 的烹调油烟作实验组 ,吸入加热空气 (2 4～ 2 8℃ )为阴性对照组 ,B(a)P气注法染毒 [12mg (kg·次 ) ,5天 1次 ]动物作阳性组。常规病理组织取材、切片 ,免疫组化法 (S -P试剂盒 )检测P53 蛋白水平。结果　阴性对照组P53 蛋白表达阴性 ;COF组有P53 蛋白表达 ,染毒时间越长 ,P53 蛋白阳性数越多 ,以细胞增生、异型增生病变组织中P53 表达阳性数为多 ;B(a)P组有P53 蛋白表达 ,但看不出时相规律。结论　烹调油烟致P53 蛋白表达增高更多还原

【Abstract】 Objective:SD rats were exposed to subacute dose cooking oil fumes(COF) in order to observe P 53 protein level of lung tissues. Methods:Rats of test group and negative group inhaled COF(43±4 mg/m 3×30 min/d×20 40,60 d) and hot air (30 min/d×20,40,60 d) respectively,while positive control group was injected Bap from trachea(12 mg/kg/5 d×20,40,60 d). An immunohistochemical study was used to investigate the expression level of P 53 . Results:In the study,the level of P 53 protein of adenic metaphasin arcad and precancerous lesion tissues is higher in the rats of test group than in the negative group. Conclution:Mutation of P 53 gene may play role in the pathological changes exposed to COF.更多还原