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培养细胞的石蜡包埋切片在凋亡研究上的运用

Application of Paraffin-embedded Section of Cultured Cell for the Study of Cell Apoptosis

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【作者】 李一琴彭军苏琦

【Author】 LI Yi-qing,PENG Jun,SU Qi (Institute of Oncology,Hengyang Medical College,Hengyang,Hunan 421001,China)

【机构】 衡阳医学院 肿瘤研究所!湖南 衡阳 421001

【摘要】 分别采用细胞涂片和细胞切片法比较 DATS 诱导的人胃癌细胞凋亡的培养细胞形态学、免疫组化及Tunel 研究效果。结果显示:细胞切片法优于细胞涂片法。细胞切片法能清晰显示细胞的结构,便于凋亡的形态学改变的观察;能准确显示免疫组化及Tunel的阳性定位,便于判断阳性结果。

【Abstract】 To investigate the morphology,immunohistochemical staining ,Tunel of human gastric cancer cell line apoptosis induced by DATS(dially trisulfide),cell s mear and paraffin-embedded section of cultured cells were applied.The results showed that paraffin-embedded section of cells clearly displayed constructu re of cells, the location of immunohistochemical staining and Tunel of cells,w hich can be easily discerned to observe the variations in cells.It was conclded that the method of paraffin-embeded section of cells is better than that of cell smear.

【关键词】 培养细胞石蜡切片凋亡
【Key words】 cultured cellparaffin-embeded sectionapoptosx
  • 【文献出处】 衡阳医学院学报(医学版) ,Journal of Hengyang Medical College , 编辑部邮箱 ,2000年05期
  • 【被引频次】3
  • 【下载频次】204
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