【摘要】 采用体外细胞培养技术和病毒体内感染模型 ,通过 Elisa、定量 PCR、流式细胞术等方法 ,研究了海洋硫酸多糖 911体外对 HIV- 1复制及体内对 SIV增殖的影响 ,并初步探讨其作用机制。结果发现 911可明显抑制HIV- 1对 MT4 细胞的急性感染和 H9细胞的慢性感染 ,其半数有效浓度 ( EC50 )分别为 4 .4 4 mg· L-1和 0 .32mg· L-1;并可明显降低猴血浆中病毒滴度及 RNA拷贝数 ,对血液中 CD4 +细胞具有一定的保护作用 ,同时可升高血液中病毒抗体的含量 ;并可明显抑制病毒逆转录酶活性 ,对 HIV- 1无明显直接灭活作用 ,但可明显干扰 HIV- 1与细胞的吸附 ,半数有效浓度 ( IC50 )为 36.51μg· L-1。提示 911体内外均可抑制艾滋病毒的增殖 ,为高效的艾滋病毒增殖抑制剂 ,其作用机制与干扰病毒与细胞吸附、抑制病毒逆转录酶活性有关。更多还原

【Abstract】 The anti AIDS effects of marine polysaccharide drug 911 both in vitro and in vivo were studied in this paper. The results indicated that 911 inhibited both acute and chronic infection of HIV 1 to MT 4 and H 9 cells with its half effect concentration (EC 50 ) at [HT5,5”] 4.44 mg·L 1 and 0.32mg·L 1 respectively in vitro. While the half toxic concentration (TC 50 ) of 911 to MT 4 cells was 7.78 kg·L 1 and that to H 9 cells 7.47g·L 1 . And the in vivo study showed that oral administration 911 reduced the virus titer and decreased plasma RNA copies in the macaques at doses of 64mg·d 1 and 320mg·d 1 . The protective effect on CD 4 + cells and antibody increasing activity of 911 were also found. Furthermore, 911 significantly interfered the adsorption of HIV to MT 4 cells and suppressed activity of reverse transcriptase. These findings indicated that 911 inhibited the viral replication both in vitro and in vivo by blocking the adsorption of virus to host cells and counteracting the activity of reverse transcriptase and modulating immune function as well.更多还原