【摘要】 采用pH漂移技术(pH drifttechnique) ,对海带雌配子体克隆的碳素营养进行了研究。结果表明 ,海带雌配子体对无机碳的利用能力较弱 ,其pH补偿点为8.85 ,CO2 补偿点为1.635μmol/L。与HCO- 3 利用有关的抑制剂 :Acetazolamide(Az) ,4′4′ diisothiocyanatosilbene 2 ,2 disulfonicacid(DIDS) ,4 acetamido 4′ isothiocyano 2 ,2′ stibene disulfonate(SITS)均未对海带雌配子体无机碳的利用产生任何影响 ,进一步说明了海带雌配子体只以游离的CO2 为无机碳源 ,不能利用HCO- 3;同时还发现 ,海带雌配子体利用无机碳的过程中 ,并未引起碱度的变化 ,从而可以用 pH指示密闭培养过程中无机碳的变化。更多还原

【Abstract】 The mechanisms and forms of inorganic carbon utilization by the female gametophytes of Laminaria japonica have been investigated by pH drift technique in a closed system. The results suggest that: female gametophytes of L.japonica have weak ability of inorganic carbon utilization, which have lower pH compensation point 8.85 but higher CO2 compen-sation point 1.635 μmol/L. Various inhibitors of HCO-3uptake: acetazolamide (Az), 4′,4′ diisothiocyanatosilbene 2,2 disulfonic acid (DIDS) and 4 acetamido 4′ isothiocyano 2,2′ stibene disulfonate (SITS) have no effect on the carbon utilization by the female gametophytes of L.japonica at different concentration of 100 μmol/L, 500 μmol/L and 1.0 mmol/L respectively, which indicate only free CO2 can be utilized by the female gametophytes of L. japonica,but HCO-3.In the course of inorganic carbon acquisition, alkality of natural seawater remains stable, so pH can be used monitoring the change of inorganic carbon in a close system.更多还原