【摘要】 目的:制备亚硒酸锰()-叶绿酸钠,并测定其对小鼠的急性毒性及对小鼠肝癌H22的抑制情况。方法:以蚕沙提取叶绿素,由叶绿素制备脱镁叶绿酸,与锰络合后制得锰()叶绿酸,再以亚硒酸化合制得亚硒酸锰()-叶绿酸钠;测定亚硒酸锰()-叶绿酸钠的急性毒性及对小鼠肝癌H22的抑制情况。结果:作者首次合成了亚硒酸锰()-叶绿酸钠。测得其LD50=1175mg·kg-1(95%的可信区间为851～1621mg·kg-1;其对小鼠肝癌H22的抑癌率为45～47%(灌胃剂量为500mg·kg-1)。结论:亚硒酸锰()-叶绿酸钠常温下性质稳定,毒性小,对小鼠肝癌H22有明显的抑制作用,可进一步做临床试验更多还原

【Abstract】 OBJECTIVE:To synthesize Sodium chlorophyllin manganese(Ⅲ) selenite(SCMS) and to test its acute toxicity and preliminary inhibiting effect on hepatoma(H 22 ) of rats.METHODS:Pheophorbin,made from chlorophyl extracted from excretion of silkworms,was coordinated with MnCl 2,and the formed manganese(Ⅲ) chlorophyllin reacted with selenous acid (H 2SeO 3)to produce SCMS.The acute toxicity of SCMS and its inhibiting effect on hepatoma (H 22 ) of rats were tested.RESULTS:The authors first synthesized SCMS.Its LD 50 =1 175 mg·kg 1 (95% confidence 851～1 621 mg·kg -1 ).Its rate of tumor inhibiting on hepatoma(H 22 ) of rats was 45～47%(dosage=500 mg·kg 1 per.os).CONCLUSION:The characteristics of SCMS is stable at normal temperature.Its toxicity is low.It is obvious that SCMS has inhibiting effect hepatoma(H 22 ) of rats.It is valuable to go further into its clinical experiment.更多还原