【摘要】 为了研究 HUMCSF1PO、HUMTPOX和 HUMTHO1基因座在云南省基诺族、佤族和布朗族中的遗传多态性分布及其在法医学中的应用价值 ,采用 PCR扩增和 PAGE变性胶电泳分离技术 ,对三个少数民族 346名无关个体及家系进行了分析 ,并将其应用于法医学实践。在这三个群体中 HU MCSF1PO、HUMTPOX和HUMTHO1基因座分别检出了 7个、8个和 6个等位基因 ,基因型频率分布均符合 Hardy- Weinberg平衡 ,三基因座累计个人识别率在 0 .9889～ 0 .9947之间 ,累计非父排除率为 0 .6 716～ 0 .7497;家系调查证实其等位基因的传递遵循孟德尔遗传规律。并对三个基因座在法医学中的应用价值也作了评价。结果提示三个基因座在三个少数民族中多态性分布较好 ,可用于法医学个人识别和亲权鉴定。更多还原

【Abstract】 This population genetic study of short tandam repeat (STR) loci HUMCSF1PO,HUMTPOX and HUMTHO1 was carried out in a sample of 344 unrelated individuals from 3 populations ( Wa, Bulang, Jinuo) living in Yunnan Province by using single PCR and PCR multiplexing followed by PAGE and silver staining. 7,8 and 6 alleles were identfied respectively on the above loci and their gene frequencies were obtained. The genotype distributions of these three loci were in accordance with Hardy Weinberg equilibirium. Family studies confirmed Mendelian inheritance of alleles. The combined discriminating power and the cumulative chance of exclusion were 0.9889 0.9947 and 0.671 0.7497 respectively. In addition, these three loci were used in forensic science practice for personal identification and parternity testing. The discrimination power and exclusion chance of these three loci are high. The methods established are sensitive and easy to perform. The results suggest that the three STR loci are useful genetic marks in forensic application in the 3 populations.更多还原