【摘要】 为探讨癌细胞淋巴管侵润转移机理，对8例直肠腺癌患者的癌周组织和转移淋巴结，以及5例正常人的直肠组织和淋巴结，采用免疫组织化学方法，检测细胞间细胞粘附分子（ICAM－1）和核转录因子KBp65（NFKBp65）的表达。同时通过地高辛碱性磷酸酶标记的寡核苷酸探针，利用原位杂交技术，对8例直肠腺癌患者的癌周组织和转移淋巴结以及5例正常人的直肠组织和淋巴结，进行ICAM－1基因的NFKB结合位点的检测。结果显示：直肠腺癌病人转移的淋巴结和癌周直肠组织中的淋巴管内皮细胞都有ICAM－1和NFKBp65表达，而在正常人的淋巴管内皮细胞无ICAM－1和NFKBp65的表达；直肠腺癌病人转移淋巴结和癌周直肠组织中的淋巴管内皮细胞核内ICAM１的启动子中存在有NFKB 结合位点。提示ICAM－1的转录，取决于可诱导的NFKB 蛋白质复合物与ICAM－1的NFKB部位结合，阻碍NFKB因子的活化能够防止癌细胞的淋巴管转移。更多还原

【Abstract】 This study inquired into the mechanism of cancer lymp hatic metastasis. The immunohistochemistry method and DNA hybridization in situ were employed in examining the peritumoral rectum tissues and metastatic lymph nodes from 8 rectu m carcinoma patients and the rectum tissues and lymph nodes from 5 normal subjec ts. The results showed that the proteins of ICAM－1 and nuclear factor KB (NF KBp65) were expressed on the lymphatic endothelial cells of the rectum adenocarcinom a patients, and there was a NFKB binding consensus sequences on ICAM－1 promot erin lymphatic endothelial cells of rectum adenocarcinoma patients. When DIG－AKP labeled 38bp oligonucleotide probe was used, there was no the expression of ICA M1 and NFKBp65 on the lymphatic endothelial cells of the normal human lymph n ode and rectum tissues. It is suggested that the activation of ICAM－1 promoter may critically depend on NFKBp65 homodimers or heterodimers binding to a variant KB site on the lymphatic endothelial cells of the rectum adencarcinoma of huma n. These observations may indicate the potential roles of NFKB in cancer metasta sis, thus giving clues to facilitate the development of a novel antimetastasis s rategy, that is obstruction the NFKBactivation pathways.更多还原