【摘要】 为探讨c-myb和bcl-2基因在C6脑胶质瘤细胞中的表达及其作用,作者采用c－myb反义硫代寡核苷酸（AON）进行裸鼠的体内试验。在裸鼠接种C6脑胶质瘤细胞株后10天，随机将动物分成正义治疗组(c－myb SON组)、反义治疗组(c－myb AON组)和对照组，每组12只。分别于治疗后第4、8、12、16天各处死动物3只，切取脑胶质瘤标本，用S－P免疫组化法观察c－myb和bcl－2蛋白的表达情况。结果：c－myb AON组c－myb蛋白和bcl－2蛋白阳性表达率与c－myb SON组和对照组比较，均有显著性差异（P<0.05）。从而提示c－myb蛋白和bcl－2蛋白可能与C6脑胶质瘤的发生、发展有关。更多还原

【Abstract】 This study was aimed at the level and implication of e xpression of c－myb and bcl－2 proteins in C6 glioma. The therapeutic effect of oncogene c－myb antisense oligodeoxynucleotide on C6 glioma in nude mice was studied. C6 gli oma cells were implanted into the cutis of 36 nude mice.Ten days later, the nude mice were randomly divided into three groups and were treated with SON, AON and normal saline respectively.Three mice of every group were killed at 4 days, 8 da ys, 12 days and 16 days after treatment, respectively.The levels of c－myb and Bcl－2 proteins expression in the three groups were observed by the S－P immunohi stochemical method. The results showed the expression of c－myb and bcl－2 protein s was significantly decreased in the AON group, compared with that in the SON and control groups (P<0.05). These data suggest that c－myb and bcl－2 proteins may play an important role in malignant glioma, and c－myb gene and bcl－2 gene may be involved in the tumorigenesis and development of glioma.更多还原