【摘要】 为建立一种检测瘤内血管生成准确定量的新方法，采用抗CD34单克隆抗体标记实体肿瘤组织中的血管内皮细胞，运用流式细胞术进行定量分析；并采用免疫组化法定量瘤内微血管密度，比较两种方法检测值的相关性。结果显示流式细胞术实验组与对照组平均检测值（％）具有显著性差异（P＜0．001）；其相关性分析提示流式细胞术内皮细胞百分比与免疫组化法微血管计数值紧密相关（CD34，r＝0．913，P＜0．001；Ⅷ因子相关抗原，r＝0．868，P＜0．001）。以上可见，流式细胞术可用于准确、快速、定量地检测实体肿瘤组织中的血管内皮细胞。更多还原

【Abstract】 Angiogenesis is essential for the growth and metastasis of solid tumors. Intratumoral microvessel count, which represents a measure of tumor angiogenesis, has been associated with prognosis of patients with a variety of malignancies. But till now, it is still a problem to quantitate the angiogenesis of tumors exactly. In this study, the endothelial cells in 20 solid tumors were conjugated with anti-CD34 monoclonal antibodies labelled with red-fluorescent substances, and were observed by fluorescence microscopy and quantitated by flow cytometry. The results of flow cytometry were analysed by t-test. The correlation was analysed between percentage of flow cytometry and vessel counts (light microscopy, ×200) in each tumor which was stained with anti-CD34 monoclonal antibodies and anti-m factor related antigen polyclonal antibodies by using immunohistochemistry. The results showed there was significant difference between the treatment group and control group in flow cytometry, and a significant correlation between the endothelia1 cel1s percentage by flow cytometry and the vessel counts by immunohistochemistry was observed, although the average vessel counts were approximately three times with CD34 staining as much as with Ⅷ factor related antigen staining. These data indicate that flow cytometry as a novel method can quantitate angiogenesis of tumors exactly and quickly.更多还原