【摘要】 动脉平滑肌细胞 (sm ooth muscle cell,SMC)的增殖在动脉粥样硬化 (atherosclerosis,AS)的形成过程中极其重要。利用体外培养的人主动脉 SMC,观察了天然高密度脂蛋白 (Native high density lipoprotein N-HDL)及氧化修饰 HDL(OX- HDL)对培养的人主动脉 SMC原癌基因 c- fos及 PDGF受体基因转录表达的影响。结果表明 :1 N- HDL 对 SMC c- fos,PDGF受体基因表达无影响 (P>0 .0 5 ) ;2 OX- HDL 对 SMC PDGF受体基因表达无影响 (P>0 .0 5 ) ;3OX- HDL使 SMC c- fos基因表达显著增强 (P<0 .0 1 ) .上述结果表明 ,OX- HDL的致 AS作用可能与刺激 SMC c- fos原癌基因表达增加有关更多还原

【Abstract】 The proliferation of arterial smooth muscle cell ( SMC) plays an important role in the process of atherosclero genesis. Using the establishment of primary culture and sub culture method for human arterial SMC, we observed the effects of native HDL (N HDL) and oxidized HDL of proto c fos and PDGF receptor genes transcriptional expression in cultured SMC. The results showed that N HDL had no effect on the expression of proto c fos and PDGF receptor genes in the cultured human SMC,that OX HDL had no effect on the expression of PDGF receptor gene in the cultured human SMC, and that OX HDL promoted the expression of protoc fos gene significantly( P <0.01). These results suggest that the OX HDL’s atherogenic role is closely related to its stimulating effect on proto c fos gene expression of SMC.更多还原