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α-萘乙酸的高效液相色谱分析
Analysis of High Performance Liquid Chromatography about α-Naphthalene Acetic Acid
【摘要】 采用高效液相色谱法 ,hypersil 5μmC1 8柱 ( 4 .6× 2 50mm) ,以pH =3~ 4的V(甲醇 )∶V(水 )∶V(磷酸 )=60∶40∶0 .35为流动相 ,检测波长 2 72nm ,测定α -萘乙酸的含量。在 0 .0 1 6~ 1 .0 0 0 g/L范围内 ,浓度与峰面积线性关系良好 (r =0 .9997) ,精密度RSD为 0 .8% (n =5) ,方法简便 ,准确。
【Abstract】 A high performance liquid chromatography method for the determination of α_naphthalene acetic acid was established on a hypersil 5 μm C 18 column(4.6×250 mm),with the mobile phase of V (methanol)∶ V (water)∶ V (phosphoric acid)=60∶40∶0.35 solution,and UV detection at 272nm.The linear correlation was observed within the range of 0.016~1 000g/L( r =0.9997).The RSD was 0.8%( n =5).
【关键词】 高效液相色谱法;
流动相;
α-萘乙酸;
【Key words】 high performance liquid chromatography; mobile phase; α-naphthalene acetic acid;
【Key words】 high performance liquid chromatography; mobile phase; α-naphthalene acetic acid;
- 【文献出处】 化学研究 ,CHEMICAL RESEARCHES , 编辑部邮箱 ,2000年01期
- 【分类号】O657.7
- 【被引频次】1
- 【下载频次】119