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高压CO2对反胶束溶解蛋白质性质的影响
Effect of High - Pressure CO2 on the Solubilization of Bovine Serum Albumin in Reverse Micelles
【摘要】 在308.15K下,研究了表面活性剂琥珀酸二(2-乙基己基)酯磺酸钠(Aerosol-OT,简称AOT)的浓度和水的含量不同时,溶解的CO2对反胶束溶解牛血清蛋白(BSA)的性质和异辛烷中AOT反胶束稳定性的影响.实验表明,在适当条件下,CO2可以使反胶束溶液中的蛋白质全部析出.本研究对有关机理进行了初步分析.
【Abstract】 The effect of dissolved CO2 on the solubilization of bovine serum albumin (BSA) in the reverse micelles of bis (2 - ethylhexyl)sulfosuccinate( AOT) in isooctane and the stability of the reverse micelles were studied at different AOT concentrations and water contents. It was found that the concentration of the surfactant and the water content in the reverse micelles significantly affected the solubilization of BSA. Most of the BSA in the reverse micelles could be precipitated under suitable conditions, and the mechanism was discussed.
【基金】 国家自然科学基金(29725308,29633020)资助项目
- 【文献出处】 化学学报 ,Acta Chimica Sinica , 编辑部邮箱 ,2000年08期
- 【分类号】O629
- 【被引频次】7
- 【下载频次】115