【摘要】 目的 :探讨蛋白激酶C(PKC)与涎腺腺样囊性癌 (SACC)发生发展的关系。方法 :采用WesternBlotting技术对PKC 5种亚型α、βⅠ 、βⅡ 、ε、δ在该肿瘤亚细胞水平的表达进行研究。结果 :①SACC及临近正常组织胞浆中均可见PKC α、PKC βⅠ 、PKC βⅡ 的表达 ,与临近正常组织相比 ,肿瘤组织胞浆中上述 3种PKC亚型表达明显降低 (P <0 0 1) ;②临近正常组织胞膜中未见PKC α的表达 ,而肿瘤组织胞膜中可见PKC α的表达 ,即PKC α在肿瘤发生发展过程中出现膜转移现象。两种组织胞膜中均无PKC βⅠ 、PKC βⅡ 的表达 ;③上述两种组织胞浆及胞膜中均未见PKC ε、PKC δ的表达。结论 :SACC的发生发展与PKC α、PKC βⅠ 、PKC βⅡ 密切相关。更多还原

【Abstract】 Objective: The protein kinase C (PKC) family of isoforms plays the key role in the regulation of cellular events, including cell proliferation, differentiation and canceration. Previous work from our laboratory has shown the alteration of PKC and its inhibitor activities in salivary adenoid cystic carcinoma (SACC) [Sun Changfu, et al. West China Journal of Stomatology, 1998,16(3):201～203]. This study aimed to further explore the relationship between PKC isoforms and canceration of SACC. Methods: Expressions of five isoforms of PKC (α、β\-Ⅰ、β\-Ⅱ、ε and δ) were examined in cytosolic and membranous fraction of specimen homogenates from SACCs and were compared with the adjacent normal tissues by immunoblotting. Equal amounts of cytosolic and membrane protein from control and experimental groups were simulaneously subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS PAGE), transferred to nitrocellulose membranes, and reacted with first and second antibodies; Color development and image analysis were performed. Results: ①Cytosolic PKC α、PKC β\-Ⅰ and PKC β\-Ⅱ were expressed in SACCs and adjacent normal tissues. The Expressions of these three cytosolic isoforms were reduced significantly in SACCs compared with the adjacent normal tissues; ②Membranous PKC α was only detected in SACCs and the membranous translocation of cytosolic PKC α was also seen. ③The expressions of PKC ε and PKC δ were not detected in both SACCs and adjacent normal tissues. Conclusion: There was close relationship between changes of expression of PKC isoforms (α、β\-Ⅰ、β\-Ⅱ) and carcinogensis of SACC. We postulated that there may be relationship between the membranous translocation of PKC α and hematogenous lung metastasis in SACC. \;更多还原